Enzyme Lab Report

Elysa Dombro
Period 5

Purpose:
      The purpose of our experiment was to test the enzyme reactions to solutions with different pH levels.

Hypothesis:
      My hypothesis was that the higher the pH level, as in the more basic, the greater the reaction of the enzymes, in this case, the amount of bubbles. 
        The independent variable is the pH levels.
        The dependent variable is the amount of bubbles.
        The controlled variables are the bindweed solution and the hydrogen peroxide.
         I chose this as my hypothesis because I think that some basic properties could cause a quicker reaction.

Materials:

• Mortar and pestle
• 5-10 grams of bindweed
• Water
• Test tubes
• ruler
• plastic beakers
• 1 mL syringe
• hydrogen peroxide
• HCI solution with pH 2-3
• HCI solution with pH 3-4
• NaOH solution with pH 10
• NaOH solution with pH 12   

Procedure:

1. Place the bindweed in the mortar and grind together as much as possible.
2. Next,  put 20 milliliters of water in the mortar with the bindweed and grind together until the bindweed is unrecognizable and becomes mush.
3. Place a paper towel over one of the plastic beaker. Pour the bindweed/water solution over the paper towel and filter out the mushy solid part from the liquid.
4. Next, use a syringe to place one milliliter of water into a test tube (this test tube should have a ruler taped onto it to the side with the millimeter side of the ruler next to the test tube).
5. Take 1 mL of the bindweed solution and add it to the water.
6. Add 1mL of hydrogen peroxide to the test tube, and record the height and time of the bubbles created by the reaction.
7. To test the rate of reaction, have one person measure the height of bubble formation and the other time the bubble growth.
8. Write down results.
9. To test the other four pH levels (HCI solution with pH of 2-3, HCI solution with pH of 3-4, NaOH solution of 10, and NaOH solution of 12), the test tubes are set up differently.
10. First put 1 mL of hydrogen peroxide in a test tube (this test tube should be set up with a ruler like the last).
11. Then and 1 mL of the bindweed solution.
12. Next, add one of the pH level solutions that you will be testing, then record your result as described in #7. Do this with all other pH level solutions.
13. Record on data and create lab report.
    
    

Data and Results:

Data Table

Seconds

0

5

10

15

20

25

30

35

40

45

50

55

pH 2/3

37 mL

37 mL

38 mL

38 mL

39 mL

39 mL

39 mL

39 mL

39 mL

40 mL

40 mL

40 mL

pH 3/4

37 mL

38 mL

39 mL

39 mL

40 mL

41 mL

42 mL

43 mL

45 mL

45 mL

45 mL

44 mL

Control

37 mL

39 mL

40 mL

40 mL

41 mL

43 mL

44 mL

45 mL

45 mL

45 mL

44 mL

41 mL

pH 10

37 mL

38 mL

40 mL

42 mL

43 mL

44 mL

44 mL

43 mL

41 mL

40 mL

38 mL

37 mL

pH12

37 mL

39 mL

42 mL

43 mL

45 mL

45 ml

40 mL

39 mL

37 mL

37 mL

37 mL

37 mL

Height of Bubbles Line Graph

______________ = control (H20, pH 7)

______________= HCI (pH 2-3)

______________= HCI (pH 3-4)

______________= NaOH (pH 10)

______________= NaOH (pH 12)

The results showed that the higher pH levels, 10 and 12, were quick to have the most amounts of bubbles. The lower pH levels, 2-3 and 3-4, took longer to build up bubbles and never reached as high an amount. The control remained in the middle of the pack. Also, after a while, the bubbles stopped growing and began to die back down, never below the original level of liquid though. The higher pH levels took less time to die down, while the lower pH levels took more time.

Conclusions:
      My hypothesis was correct, because I hypothesized that the higher the pH level, the greater reaction there would be. I found that with the higher pH, the bubbles grew much quicker and grew in height more than the lower pH levels, but died down again quickly while the lower pH levels took longer to subside.After doing research, I better understood that the bubbles I saw were the reactions of the hydrogen peroxide and enzymes creating oxygen bubbles. I think the basic solutions reacted quicker because they reacted with the slightly acidic hydrogen peroxide to create a slightly quicker reaction. The bases created more hydrogen bubbles.