Teo Martinez
Per. 5
Nathan, Matt and I wanted to test the reactions of enzymes with solutions of different pH levels. We had ten test tubes and did two trials of the experiment to double-check our results.
Materials
10-40 mL test tubes labeled #1-10
8-Test Tube stands/rack
3-1 mL pipette
3- small plastic beakers
1-pastel
~8-paper towels
1-ruler
1-stopwatch
2-markers
Hypothesis
If the reaction of an enzyme relates to the acidity of a solution, then more acidic solutions will react more.
Procedure
Put 5 mL of different pH level solutions into 8 of the 10 test tubes and leave the last 2 empty. After taking note of which solutions are where, take the first test tube and add the enzyme solution. Mark where the liquid level is, then add the Hydrogen Peroxide. Every 2 seconds make a mark of where the reaction’s bubbles have risen to. Do this for 30 seconds. After the reaction is finished, measure where each line is in relation to the starting liquid level. Repeat these steps with the remaining test tubes then record your results.
Results
First Trial:
2 pH Solution Test 1 (cm)
|
3.5 pH Solution Test 1 (cm)
|
7 pH Solution Test 1 (cm)
|
10 pH Solution Test 1 (cm)
|
12 pH Solution Test 1 (cm)
| |
2"
|
0
|
0
|
0
|
0
|
0
|
4"
|
0
|
0
|
0
|
0
|
0
|
6"
|
0
|
0
|
0
|
0
|
0
|
8"
|
0
|
0
|
0.1
|
0
|
0
|
10"
|
0
|
0
|
0.3
|
0
|
0.1
|
12"
|
0
|
0
|
0.4
|
0.1
|
0.1
|
14"
|
0
|
0.1
|
0.6
|
0.2
|
0.2
|
16"
|
0
|
0.2
|
0.7
|
0.4
|
0.2
|
18"
|
0
|
0.2
|
0.7
|
0.6
|
0.2
|
20"
|
0
|
0.2
|
0.8
|
0.7
|
0.2
|
22"
|
0
|
0.3
|
0.9
|
0.8
|
0.2
|
24"
|
0
|
0.4
|
1
|
0.9
|
0.2
|
26"
|
0
|
0.4
|
1.3
|
1
|
0.3
|
28"
|
0
|
0.5
|
1.4
|
1
|
0.4
|
30"
|
0.2
|
0.6
|
1.6
|
1.1
|
0.5
|
Second Trial:
2 pH Solution Test 2 (cm)
|
3.5 pH Solution Test 2 (cm)
|
7 pH Solution Test 2 (cm)
|
10 pH Solution Test 2 (cm)
|
12 pH Solution Test 2 (cm)
| |
2"
|
0
|
0
|
0
|
0
|
0
|
4"
|
0
|
0
|
0
|
0
|
0
|
6"
|
0
|
0
|
0
|
0
|
0
|
8"
|
0
|
0.1
|
0
|
0
|
0
|
10"
|
0
|
0.1
|
0.1
|
0
|
0
|
12"
|
0
|
0.1
|
0.1
|
0.1
|
0
|
14"
|
0
|
0.2
|
0.3
|
0.1
|
0
|
16"
|
0.1
|
0.2
|
0.3
|
0.1
|
0.1
|
18"
|
0.1
|
0.3
|
0.4
|
0.2
|
0.1
|
20"
|
0.1
|
0.3
|
0.4
|
0.2
|
0.1
|
22"
|
0.1
|
0.3
|
0.4
|
0.3
|
0.1
|
24"
|
0.1
|
0.4
|
0.5
|
0.3
|
0.1
|
26"
|
0.1
|
0.4
|
0.5
|
0.4
|
0.1
|
28"
|
0.1
|
0.4
|
0.6
|
0.4
|
0.2
|
30"
|
0.1
|
0.5
|
0.7
|
0.5
|
0.2
|
Conclusion
Apparently the more extreme the pH, whether in acidity or basicness, the less it reacts than water, which has a stable pH of 7. In both trials, the 2 pH solution reacted the least. My hypothesis was incorrect, since the acidic solutions reacted down very little. However, it is not surprising that the water reacted most, since enzymes are useful in our bodies, which are made up of 80% water.
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ReplyDeleteEnzyme Lab e-Report Evaluation Summary: Teo M
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Title: 2/2 ()
Introduction: 2/2 ()
Purpose: 2/2 (Stated in intro)
Hypothesis: 1.5/2 ("If the reaction of an enzyme relates to the acidity of a solution, then more acidic solutions will react more..." vigorously? Rapidly??)
Materials: 2/2 ()
Procedure: 7/10 (No explanation of how to prepare extract. Remaining instructions could have been more explicit.)
Observations/Data: 10/10 ()
Data Analysis: 1/10 (virtually none, no graph or talk of statistical analysis [averaging etc.])
Discussion: 3/10 (Very limited--problems encountered in this experiment? Future experiments?)
TOTAL: 30.5/50
COMMENTS: